RNASEH2A monoclonal antibody (M01), clone 3G5-F5
产品名称： RNASEH2A monoclonal antibody (M01), clone 3G5-F5
英文名称： RNASEH2A monoclonal antibody (M01), clone 3G5-F5
产品编号： H00010535-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a full length recombinant RNASEH2A.
- Immunogen:
- RNASEH2A (AAH11748, 1 a.a. ~ 299 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MDLSELERDNTGRCRLSSPVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERERLFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQVFVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEKLQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWEDSASENQEGLRKITSYFLNEGSQARPRSSHRYFLERGLESATSL
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (58.63 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Cell lysate)
- RNASEH2A monoclonal antibody (M01), clone 3G5-F5 Western Blot analysis of RNASEH2A expression in Jurkat ( Cat # L017V1 ).
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of RNASEH2A expression in transfected 293T cell line by RNASEH2A monoclonal antibody (M01), clone 3G5-F5.
Lane 1: RNASEH2A transfected lysate(33.4 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged RNASEH2A is 1 ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of RNASEH2A over-expressed 293 cell line, cotransfected with RNASEH2A Validated Chimera RNAi ( Cat # H00010535-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNASEH2A monoclonal antibody (M01), clone 3G5-F5 (Cat # H00010535-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 10535
- GeneBank Accession#:
- BC011748
- Protein Accession#:
- AAH11748
- Gene Name:
- RNASEH2A
- Gene Alias:
- AGS4,JUNB,RNASEHI,RNHIA,RNHL
- Gene Description:
- ribonuclease H2, subunit A
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid
- Other Designations:
- ribonuclease H2, large subunit,ribonuclease HI, large subunit
- Gene Pathway