分子量:47.8 kDa

纯度:>85% as determined by reduced SDS-PAGE. 

内毒素:Less than 1.0 EU per μg of the Human Testican 1, His Tag by the LAL method.

Buffer:20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4.

生物活性:Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC.

产品特性:Human Testican 1, His Tag is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 47.8 kDa. The predicted N-terminus is Arg 22. The reducing (R) protein migrates as 50-60 kDa in SDS-PAGE  due to glycosylation.

产品背景:Testican-1 is also known as protein SPOCK，SPOCK1, which contains one azal-like domain and one thyroglobulin type-1 domain.SPOCK1 is an extracellular heparan/chondroitin sulfate proteoglycan. Members of this family are known as testicans, also called SPOCKs. SPOCKs are enriched in brain and have been shown to regulate neuronal attachment and outgrowth. They contain inhibitory regions in several domains targeted to different classes of protease, and in some cases may act as protease inhibitors. Little is known about SPOCK1's function until now. It may play a role in cell-cell and cell-matrix interactions except for contributing to various neuronal mechanisms in the central nervous system.