PNCK (Human) Recombinant Protein
产品名称: PNCK (Human) Recombinant Protein
英文名称: PNCK (Human) Recombinant Protein
产品编号: P5503
产品价格: 0
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围: null
亚诺法生技股份有限公司(Abnova)
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- Specification
- Product Description:
- Human PNCK (Q6P2M8, 1 a.a. - 343 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
- Theoretical MW (kDa):
- 66
- Form:
- Liquid
- Preparation Method:
- Baculovirus infected insect cell (Sf21) expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 62 % by SDS-PAGE/CBB staining
- Activity:
- The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate, Mg(or Mn)/ATP, and Ca/Calmodulin . The phosphorylated and unphosphorylated substrates were separated and detected by LabChip TM3000. Substrate: Synapsin peptide. ATP: 100 uM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Loading 1 ug protein in SDS-PAGE
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 139728
- Protein Accession#:
- Q6P2M8
- Gene Name:
- PNCK
- Gene Alias:
- BSTK3,CaMK1b,FLJ50403,FLJ50549,FLJ56451,FLJ59811,MGC45419
- Gene Description:
- pregnancy up-regulated non-ubiquitously expressed CaM kinase
- Omim ID:
- 300680
- Gene Ontology:
- Hyperlink
- Gene Summary:
- PNCK is a member of the calcium/calmodulin-dependent protein kinase family of protein serine/threonine kinases (see CAMK1; MIM 604998) (Gardner et al., 2000 [PubMed 10673339]).[supplied by OMIM
- Other Designations:
- OTTHUMP00000025964,pregnancy upregulated non-ubiquitously expressed CaM kinase