CDC2 (phospho S39) Peptide
产品名称： CDC2 (phospho S39) Peptide
英文名称： CDC2 (phospho S39) Peptide
产品编号： P1296
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC2.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S39 of human CDC2.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase.
Kramer A, Mailand N, Lukas C, Syljuasen RG, Wilkinson CJ, Nigg EA, Bartek J, Lukas J.Nat Cell Biol. 2004 Sep;6(9):884-91. Epub 2004 Aug 15.
- 2.
- Keratinocyte G2/M growth arrest by 1,25-dihydroxyvitamin D3 is caused by Cdc2 phosphorylation through Wee1 and Myt1 regulation.
Dai X, Yamasaki K, Yang L, Sayama K, Shirakata Y, Tokumara S, Yahata Y, Tohyama M, Hashimoto K.J Invest Dermatol. 2004 Jun;122(6):1356-64.
- 3.
- Mitotic phosphorylation of the peripheral Golgi protein Nir2 by Cdk1 provides a docking mechanism for Plk1 and affects cytokinesis completion.
Litvak V, Argov R, Dahan N, Ramachandran S, Amarilio R, Shainskaya A, Lev S.Mol Cell. 2004 May 7;14(3):319-30.
- Applications
- Western Blot (Cell lysate)
- CDC2 (phospho S39) polyclonal antibody (Cat # PAB0436). Western blot analysis of CDK1 expression in Hela NE.
- Protocol Download
- Western Blot (Cell lysate)
- The CDC2 (phospho S39) polyclonal antibody (Cat # PAB0436) is used in Western blot for detection in Ramos cell lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CDC2 (phospho S39) polyclonal antibody (Cat # PAB0436) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 983
- Protein Accession#:
- NP_001777;P06493
- Gene Name:
- CDC2
- Gene Alias:
- CDC28A,CDK1,DKFZp686L20222,MGC111195
- Gene Description:
- cell division cycle 2, G1 to S and G2 to M
- Omim ID:
- 116940
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- OTTHUMP00000019660,cell cycle controller CDC2,cell division control protein 2 homolog,cell division cycle 2 protein,cyclin-dependent kinase 1,p34 protein kinase
- Gene Pathway