分子量:31.3 kDa

纯度:>90% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human TIM-1, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its ability to inhibit antiCD3induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and rhTIM1. The ED50 for this effect is typically 0.15-1.0 μg/mL.

产品特性:Human TIM-1, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 31.3 kDa. The predicted N-terminus is Ser 21 . The reducing (R) protein migrates as 80-100 kDa in SDS-PAGE  due to glycosylation.

产品背景:Hepatitis A virus cellular receptor 1 is also known as HAVCR1, HAVCR, KIM1, TIM, TIM1, TIMD1,  is widely expressed with highest levels in kidney and testis. The protein encoded by HAVCR1 gene is a membrane receptor for both human hepatitis A virus (HHAV) and TIMD4. The encoded protein may be involved in the moderation of asthma and allergic diseases. The reference genome represents an allele that retains a MTTVP amino acid segment that confers protection against atopy in HHAV seropositive individuals. Three transcript variants encoding the same protein have been found for this gene. HAVCR1 may play a role in T-helper cell development, the regulation of asthma and allergic diseases and in kidney injury and repair. In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus.