phospho-CaMK2 beta/gamma/delta (Thr287),磷酸化钙/钙调素依赖蛋白激酶CAMK2B/D/G抗体

产品编号HZ-5282R
英文名称phospho-CaMK2 beta/gamma/delta (Thr287)
中文名称磷酸化钙/钙调素依赖蛋白激酶CAMK2B/D/G抗体
别 名CaMK2 beta gamma delta (phospho Thr287); CaMK2 beta gamma delta (phospho T287); CaMK2 beta; CaMK2 gamma; CaMK2 delta; CAMK2G; Calcium/calmodulin dependent protein kinase (CaM kinase) II beta; Calcium/calmodulin dependent protein kinase II beta; Calcium/calmodulin dependent protein kinase IIB; Calcium/calmodulin dependent protein kinase type II beta chain; CAM 2; CaM kinase II beta chain; CaM kinase II beta subunit; CAM2; CAMK 2; CAMK 2B; CaMK II beta subunit; CaMK II subunit beta; CAMK2; CaMK2 beta; CAMK2B; CAMKB; CaMKII beta subunit; CaMKIIB; MGC29528; Proline rich calmodulin dependent protein kinase; CaM kinase II; Calcium/calmodulin-dependent protein; CaM kinase II gamma subunit; CaM-kinase II gamma chain; CaM kinase II delta subunit; CaM-kinase II delta chain; CAM2; CAMK; CAMK-II; CAMK2D; CAMK2G; CAMK2N2; CAMKD; CAMKG; KCC2B; kinase CaMK2-beta; KCC2B_HUMAN.
说 明 书0.1ml
产品类型磷酸化抗体
phospho-CaMK2 beta/gamma/delta (Thr287),磷酸化钙/钙调素依赖蛋白激酶CAMK2B/D/G抗体研究领域肿瘤 免疫学 神经生物学 转录调节因子 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Pig, Horse, Rabbit, Guinea Pig,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量61kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human CAMK2G around the phosphorylation site of Ter286 [QE(p-T)VE]
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-CaMK2 beta/gamma/delta (Thr287),磷酸化钙/钙调素依赖蛋白激酶CAMK2B/D/G抗体PubMedPubMed
产品介绍background:
The multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Autophosphorylation plays an important role in the regulation of the kinase activity. CaMKII is required for synaptic plasticity, as in Long Term Potentiation (LTP), a cellular model for learning and memory.

Function:
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplamic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplamic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning.

Subunit:
CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other.

Subcellular Location:
Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (Probable).

Tissue Specificity:
Expressed in skeletal muscle.

Post-translational modifications:
Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.

Similarity:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.

Gene ID:
816

phospho-CaMK2 beta/gamma/delta (Thr287),磷酸化钙/钙调素依赖蛋白激酶CAMK2B/D/G抗体Database links:
Entrez Gene: 525416 Cow
Entrez Gene: 816 Human
Entrez Gene: 12323 Mouse
Entrez Gene: 24245 Rat
Entrez Gene: 399284 Xenopus laevis
Omim: 607707 Human
SwissProt: Q3MHJ9 Cow
SwissProt: Q13554 Human
SwissProt: P28652 Mouse
SwissProt: P08413 Rat
Unigene: 351887 Human
Unigene: 439733 Mouse
Unigene: 9743 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CaMK2b也是丝/苏氨基酸激酶的一种，有称CAMK2G，同属于Ca++/钙调素依赖性蛋白质激酶家族成员。钙／钙调素依赖性蛋白激酶Ⅱb（CaMKⅡb）广泛分布于中枢神经系统内，参与调节神经递质的合成释放、信号转导和突触可塑性的形成以及磷酸化蛋白。

产品图片

Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-phospho-CaMK2 beta/gamma/delta (Thr287) Polyclonal Antibody, Unconjugated (HZ-5282R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining