分子量:71.2 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg by the LAL method.

Buffer:PBS, pH7.4

生物活性:(1) Measured by its binding ability in a functional ELISA against immobilized Human EGF, Tag Free (Cat# EGF-H4114, 0.5 μg/well). The linear range is 0.6-10 μg/mL.
(2) Measured by its binding ability in a functional ELISA against immobilized CetuxiMab (0.2 μg/well). The linear range is 0.4-12.5 ng/mL.
(3) Measured by its ability to bind Human EGF, Tag Free (Cat# EGF-H4114) in the SPR assay (Biacore 2000) with an estimated KD of 120nM.

产品特性:This protein carries an Avi tag (Avitag) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 71.2 kDa. The protein migrates as 110-115 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

产品背景:The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.