EIF2S3抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
EIF2S3抗体

EIF2S3抗体

商家询价

产品名称： EIF2S3抗体

英文名称： EIF2S3Antibody

产品编号： XY11162-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 EIF2S3抗体

Applications
In-house applications ELISA, WB, IHC, IP, IF     Application not tested? Please see our Guarantee.
Published applications WB Publications 1 citations –view here
Species specificity Human, Mouse; other species not tested.
Published Species Human
Images
Prev12NextView more validation data
  • 11162-1-AP;HeLa cellsHeLa cells were subjected to SDS PAGE followed by western blot with 11162-1-AP(EIF2S3 antibody) at dilution of 1:1000
  • 11162-1-AP;human lung cancerImmunohistochemical of paraffin-embedded human lung cancer using 11162-1-AP(EIF2S3 antibody) at dilution of 1:50 (under 10x lens)
  • 11162-1-AP;HeLa cellsIP Result of anti-EIF2S3 (IP:11162-1-AP, 4ug; Detection:11162-1-AP 1:500) with HeLa cells lysate 2800ug.
  • 11162-1-AP;HeLa cellsImmunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 11162-1-AP( EIF2S3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Positive WB detected in HeLa cells, COLO 320 cells, mouse thymus tissue
Positive IP detected in HeLa cells
Positive IHC detected in Human lung cancer tissue, human tonsillitis tissue
Positive IF detected in HeLa cells
Recommended dilution WB : 1:500-1:5000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:50-1:500
 EIF2S3抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen EIF2S3 fusion protein ag1650 Full name Eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
Calculated molecular
weight		 52 kDa Observed molecular 
weight		 52 kDa
GenBank accession 
number		 BC019906 Gene ID (NCBI) 1968
Gene symbol EIF2S3 Synonyms EIF 2 gamma X, eIF 2gA, eIF 2gX, EIF2, EIF2G, EIF2gamma, EIF2S3
Background EIF2S3抗体

Interacts with GTP and initiator methionyl-tRNA, translation initiation factor eIF2 forms a ternary complex that binds the 40S ribosome and then scans an mRNA to select the AUG start codon for protein synthesis[PMID: 9736774]. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2S3 is the core subunit of the heterotrimeric eIF2 complex.[PMID:23063529] EIF2S3抗体

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