phospho-Ephexin-1 (Tyr87),磷酸化神经细胞鸟苷酸置换因子Ephexin1抗体

产品编号HZ-14610R
英文名称phospho-Ephexin-1 (Tyr87)
中文名称磷酸化神经细胞鸟苷酸置换因子Ephexin1抗体
别 名Ephexin-1 (phospho Y87); p-Ephexin-1 (phospho Y87); ARHGEF27; Eph-interacting exchange protein; EPHEXIN; Ephexin 1; Neuronal guanine nucleotide exchange factor; Ngef; NGEF_HUMAN.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域细胞生物 信号转导 G蛋白偶联受体 G蛋白信号
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat,
phospho-Ephexin-1 (Tyr87),磷酸化神经细胞鸟苷酸置换因子Ephexin1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量82kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Ephexin-1 around the phosphorylation site of Tyr87
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-Ephexin-1 (Tyr87),磷酸化神经细胞鸟苷酸置换因子Ephexin1抗体PubMedPubMed
产品介绍background:
EPHEXIN is a 710 amino acid protein that localizes to both the membrane and the cytoplasm and contains one SH3 domain, one PH domain and one DH domain. Expressed at high levels in brain and present at lower levels in lung tissue, EPHEXIN interacts with EphA4 and functions as a guanine nucleotide exchange factor (GEF) that is capable of activating Rho A, Rac 1 and Cdc42 and is thought to play a role in axon guidance and growth cone collapse. EPHEXIN is subject to Src-dependent phosphorylation, an event that increases the GEF activity of EPHEXIN toward Rho A. Human EPHEXIN, which exists as multiple alternatively spliced isoforms, shares a high degree of sequence homology with its mouse counterpart, suggesting a conserved role between species.

Function:
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension.

Subunit:
Interacts with CDK5R1 and EPHA4; activated by EPHA4 through the CDK5 kinase.

Subcellular Location:
Cytoplasm. Membrane. Cell projection, growth cone. Associated with membranes. Localizes to axonal growth cones.

phospho-Ephexin-1 (Tyr87),磷酸化神经细胞鸟苷酸置换因子Ephexin1抗体Tissue Specificity:
Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.

Post-translational modifications:
Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA.

Similarity:
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
Database links : UniProtKB/Swiss-Prot: Q8N5V2.2

Gene ID:
424744

Database links:
Entrez Gene: 424744 Chicken
Entrez Gene: 25791 Human
Entrez Gene: 53972 Mouse
Entrez Gene: 246217 Rat
Omim: 605991 Human
SwissProt: Q8N5V2 Human
SwissProt: Q8CHT1 Mouse
SwissProt: Q5BKC9 Rat
Unigene: 97316 Human
Unigene: 435439 Mouse
Unigene: 20670 Rat

phospho-Ephexin-1 (Tyr87),磷酸化神经细胞鸟苷酸置换因子Ephexin1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.