DACH1抗体
Applications
| In-house applications |
ELISA, IHC, IP, WB, IF Application not tested? Please see our Guarantee. |
| Published applications |
ChIP, IF, IHC, WB |
Publications |
21 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Human, mouse, rat |
|
Images
Prev12Next
 HEK293 cells (Native, siRNA-control, siRNA-DACH1) were subjected to SDS PAGE followed by western blot with 10914-1-AP (DACH1 antibody) at dilution of 1:1000 IP Result of anti-DACH1 (IP:10914-1-AP, 4ug; Detection:10914-1-AP 1:1000) with HEK-293 cells lysate 6000ug. Immunohistochemical of paraffin-embedded human prostate cancer using 10914-1-AP(DACH1 antibody) at dilution of 1:50 (under 10x lens) HEK-293 cells were subjected to SDS PAGE followed by western blot with 10914-1-AP( DACH1 Antibody) at dilution of 1:600 Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HEK-293 cells using 10914-1-AP( DACH1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
|
| Positive WB detected in |
HEK-293 cells, HEK-293 |
| Positive IP detected in |
HEK-293 cells |
| Positive IHC detected in |
Human prostate cancer tissue |
| Positive IF detected in |
HEK-293 cells |
| Recommended dilution |
WB : 1:200-1:2000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:20-1:200 |
DACH1抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
DACH1 fusion protein ag1354 |
Full name |
Dachshund homolog 1 (Drosophila) |
Calculated molecular
weight |
79 kDa |
Observed molecular
weight |
97-100 kDa |
GenBank accession
number |
BC021219 |
Gene ID (NCBI) |
1602 |
| Gene symbol |
DACH1 |
Synonyms |
DACH, DACH1, Dachshund homolog 1, Dachshund-1 |
Background DACH1抗体
|
DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1. DACH1抗体
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