分子量:46.1 kDa

纯度:>92% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Influenza A [A/Thailand/1(KAN-1)/2004 (H5N1)] NA by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at pH 7.5 at 37℃.

产品特性:Influenza A [A/Thailand/1(KAN-1)/2004 (H5N1)] NA is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 46.1 kDa. The predicted N-terminus is His 36. The reducing (R) protein migrates as 48 kDa in SDS-PAGE .

产品背景:Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.