PRPF3抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
PRPF3抗体

PRPF3抗体

商家询价

产品名称： PRPF3抗体

英文名称： PRPF3Antibody

产品编号： XY 10106-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
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 PRPF3抗体Applications

In-house applications ELISA, WB, IP     Application not tested? Please see our Guarantee.
Published applications WB Publications 2 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human
Images
Prev1NextView more validation data
  • 10106-1-AP;Y79 cellY79 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP(PRPF3 antibody) at dilution of 1:1000
  • 10106-1-AP;mouse liver tissueIP Result of anti-PRPF3 (IP:10106-1-AP, 5ug; Detection:10106-1-AP 1:500) with mouse liver tissue lysate 4000ug.
Positive WB detected in Y79 cells, HepG2 cells, L02 cells, mouse brain tissue, mouse liver tissue
Positive IP detected in Mouse liver tissue
Recommended dilution WB : 1:500-1:5000
IP : 1:200-1:2000
 PRPF3抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen PRPF3 fusion protein ag0154 Full name PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae)
Calculated molecular
weight		 78 kDa Observed molecular 
weight		 90 kDa or 78 kDa
GenBank accession 
number		 BC000184 Gene ID (NCBI) 9129
Gene symbol PRPF3 Synonyms HPRP3, HPRP3P, Pre mRNA splicing factor 3, PRP3, Prp3p, PRPF3, RP18, U4/U6 snRNP 90 kDa protein
Background PRPF3抗体

The pre-mRNA splicing occurs in spliceosomes, which consist of four ribonucleoprotein (snRNP) particles (U1, U2, U5 and U4/U6) and more than fifty proteins. This U4/U6-associated splicing factor, Hprp3p, is a 77 kDa protein, which is homologous to the Saccharomyces cerevisiae splicing factor Prp3p and may play a role in spliceosome assembly. Western blot analysis of HeLa cell nuclear extracts detected PRP3 protein at an apparent molecular mass of about 90 kD(PMID: 9328476 ). The discrepancy between the molecular mass reported here and that predicted from the Hprp3p coding sequence can be attributed, at least partially, to the highly positive charge; its value of predicted isoelectric point (IP) is ∼9.99. This antibody is a rabbit polyclonal antibody raised against an internal region of human PRPF3. PRPF3抗体

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