PRDX2 Rabbit Polyclonal Antibody (KO Validated)-抗体-抗体-生物在线
上海雅吉生物科技有限公司
PRDX2 Rabbit Polyclonal Antibody (KO Validated)

PRDX2 Rabbit Polyclonal Antibody (KO Validated)

商家询价

产品名称: PRDX2 Rabbit Polyclonal Antibody (KO Validated)

英文名称: PRDX2 Rabbit Polyclonal Antibody (KO Validated)

产品编号: K5273

产品价格: null

产品产地: 上海

品牌商标: 雅吉生物

更新时间: null

使用范围: WB, IF, ICC

上海雅吉生物科技有限公司
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来源用途交叉反应性理论分子量实际分子量
RabbitWB, IFH, M15/21kDa20kDa

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WBIPIFIHCICCFCChIPChIP-seq
1:500-1:2000-1:50-1:200-----

抗体详细信息如下::

About this Antibody
NamePRDX2 Rabbit Polyclonal Antibody (KO Validated)
CategoryRabbit Polyclonal Antibody (pAb); Primary antibody
IsotypeIgG
Purification methodAffinity purification
Positive samplesSW480, 22Rv1, HepG2, K-562, BT-474, Mouse brain, Mouse kidney
Cellular locationCytoplasm
Customer validation-
About the Immunogen
ImmunogenRecombinant fusion protein of human PRDX2 (NP_859428.1).
SequenceMASGNARIGKPAPDFKATAVVDGAFKEVKLSDYKGKYVVLFFYPLDFTFVCPTEIIAFSNRAEDFRKLGCEVLGVSVDSQFTHLAWYEQGPKREVAAKLTPSGPSSVASWPLLNLWNLRFPIVKIMETLPPKSLRMMTVISI
Gene ID7001
Swiss ProtP32119
SynonymsPRDX2; HEL-S-2a; NKEF-B; NKEFB; PRP; PRX2; PRXII; PTX1; TDPX1; TPX1; TSA; peroxiredoxin-2
CategoryToll-Like Receptor Signaling; T-Cell Receptor Signaling
BackgroundPeroxiredoxin 2 (PRDX2, PRXII, NKEFB) is a ubiquitously expressed thioredoxin peroxidase. The enzyme catalyzes the reduction of hydrogen peroxide and organic hydroperoxides via the thioredoxin system. An antioxidant, PRDX2 neutralizes endogenous reactive oxygen species (ROS) and regulates cytokine-induced peroxide levels for normal cell function. Research studies have shown that PRDX2 plays important roles in inflammation, cancer, and natural killer (NK) cell activation. During cancer progression, PRDX2 is upregulated and protects cancer cells from oxidative stress-induced apoptosis. In inflammatory diseases such as infection, myocardial infarction, and ischemia, PRDX2 not only protects (host) cells from oxidative stress-induced death, but is also released into extracellular space to trigger local inflammation and to activate NK cells for innate immune response.

包装清单:

产品编号产品名称包装
AF5273PRDX2 Rabbit Polyclonal Antibody (KO Validated)50μl
AZ050Western一抗稀释液50ml
说明书1份

保存条件:
PRDX2 Rabbit Polyclonal Antibody (KO Validated) -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

代表性图片:

Fig. 1. Western blot analysis of extracts of various cell lines, using PRDX2 antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231).Fig. 2. Western blot analysis of extracts from normal (control) and PRDX2 knockout (KO) 293T cells, using PRDX2 antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s.
Fig. 3. Immunofluorescence analysis of HeLa cells using PRDX2 antibody.